Reversed Phase - Introduction and Peptide Level Analysis

This modules introduces the use of reversed phase HPLC for the characterization of biopharmaceuticals at the peptide level.

Topics include:

• Introduction to reversed phase HPLC
• Analysis at the peptide level
• mAB peptide map
• Common mAb post-translational modifications (PTMs)
• Glycoprofile
• Deamidation
• Oxidation
• Pyroglutamate formation
• Peptide mapping in a routine environment
• Disulfide bridge reduction
• Comparison of digestion enzymes